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Following the blocking of membrane with 5% non-fat milk in TBST for 30 minutes, the membranes were washed three times with TBST for 10 minutes each, and the membranes were incubated in primary antibodies at a 1:1000 dilution in 2% milk overnight at 4C

doi: 10.1016/j.freeradbiomed.2015.06.006 Gorelenkova Miller O., Mieyal J

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4 However, a separate medical records analysis suggested that non-diabetic men using semaglutide were slightly more likely to develop low testosterone than those who werent